Generatx, is a group of physicians who share the philosophy of treating patients with “their own” defense, they have created and evaluated a customized program, using and evaluating the molecular profile of the patient’s own cells to slow down and eradicate the disease.
Introduction to the Cancer Treatment Program
At the time of proposing a targeted treatment for patients with cancer it should be taken into account all the other aspects that surround or influence the progression and tumor cell generation, conventional treatments, they just destroy tumor burden, but in some instances, this process alters the environment around the tumor and may promote cell embolization beyond the destruction of the tumor mass, and which result in metastatic disease with chemo or radio resistance, making it more difficult to treat, however if these elements are taken into account and therapies are more personalized and targeted, the expected result should be the control of tumor progression and improved survival of patients with cancer.
1.1. Summary of the Therapy Cancer Therapy
This is our full immunotherapy treatment for cancer patients. It takes 17 days, you will stay at 5 stars hotel and will take treatment at mornings in our center
1.2. Patient Evaluation
Once patients have been admitted to the program are evaluated personally by an internist, a dietician and physician to carry out the planning and monitoring of the program.
EVALUATION phase, where we review the patient, not only physically but metabolically and molecularly, and we take special interest in the diagnosis of other conditions, which may alter or accelerate the healing process.
1.2.1. Medical Evaluation
Patients admitted to the Cellular Immunotherapy Program are evaluated medically and biochemically, this done by an internist, with the aim of identifying and correcting the risk factors affecting the survival and healing process of patients, such as infectious processes and risk of thrombosis, all patients have a nutritional assessment conducted for patients with cancer.
In this way patients are evaluated from a thorough point of view and not only from the point of view or Cancer or tumor.
22.214.171.124 Medical Record review
126.96.36.199 Evaluation by Internal Medicine Team
188.8.131.52 Nutritional Evaluation
1.2.2. Basic Test
Patients are evaluated from a biochemically point, for this purpose, a number of basic studies will be done to evaluate the metabolic function, hematological function and coagulation, as well as the measurement of parameters to evaluate the risk of thrombosis in patients.
184.108.40.206 RED AND WHITE CELL COUNT
220.127.116.11 SMAC (TOTALCHEMESTY TEST)
18.104.22.168 Urine Test
22.214.171.124 Coagulation Times
126.96.36.199 Tumor Marker
1.2.3. Directed Studies:
Tumor Necrosis Factor
This cytokine has been linked to the progression of the disease, and the probability of the existence of metastases in cancer patients, their control is part of the treatment.
Counting of Circulating Tumor Cells (CTC)
Circulating tumor cells is the test that evaluates the response to our treatment, and that leads us to an assessment of the severity of the disease, its control and reduction is one of our goals.
Regulatory T lymphocytes cells
Lymphocytes T are a type of regulatory lymphocytes that counteract or inactivate the “activation” of the immune system, there is a direct relationship between these cells and the progression of the disease, and that has been associated with failure in some programs of immunotherapy.
Is an essential antioxidant, plays an important role in controlling the population of leukocytes, their loss is related to the use of radiation, stress, aging, certain drugs and contamination affecting the immune cells, its balance is an important part of our program.
Sensitivity to alternative health products and complementary medicine
The customization of therapy is one of our goals, natural products have been shown to have beneficial effects in patients with cancer, but the abuse of them may affect liver detoxification system, plus it could have an “antagonistic” effect, that is, that some of the drugs can suppress the effect of other, neutralizing the effect of both drugs, canceling their effectiveness, the ideal is the combination of these products that demonstrate more effectiveness in the mortality malignant cells, and on the other hand those who prove to have greater impact on the population of white cells of the immune system.
The various natural products that have proven effect on mortality of cancer cells in vitro, are evaluated for each patient to customize the complementary therapy, as we can see from the chart, circulating tumor cells were incubated 24 hours with different products listed in the horizontal, the results are different, in this particular case, the patient should not be treated with products that were lower than 85% effective.
Patients are evaluated with a panoramic radiograph, which will give us a more detailed and accurate evaluation of the patient’s dental condition, we also help detect cavitation or other sources of infection.
Another infectious process no less common in patients with cancer, are infections of the respiratory system, so a chest radiograph may help us in this diagnosis, patients are evaluated also with a CT scan, this study is at the discretion of the physician who evaluates the patient during the intake process.
188.8.131.52 Panoramic X-Ray
184.108.40.206 MRI/CT scan as indicate and required
During the evaluation of the internist it is included an electrocardiographic evaluation in order to detect heart problems that may affect the survival of patients and may guide us towards its treatment and prevention.
1.3. Conditioning and Preparation
Our second phase is CONDITIONING, at this stage the protein deficiencies are corrected, specific nutrients are provided those that demonstrate in vitro effect on mortality of malignant cells, and on the other hand favoring the activation of the immune system, during this stage favorable detoxification procedure is also performed, infectious foci are eliminated and diminished the blocking cells of the immune response,
One phase of our program is called DETOX, is the initial part of it and the goal is to improve (change) the tumor microenvironment.
DIET FOR PATIENTS WITH CANCER
Diet is an important factor in the etiology of various types of cancer.
Its effect has been extensively studied from the metabolic and epidemiological point of view. Dietary factors studied cover from macro-nutrients like fats to micro-nutrients such as antioxidants substances.
Neoplasms have their origin on two factors: genetic or endogenous and the environmental or exogenous. In fact, the environmental factors are responsible for 98% of all tumors.
The environmental carcinogenic agents are classified as chemical, biological and physical. It will be their concentration or level of exposure and time of action what will determine the possible pathological effects at a short or long term.
Among the Environmental factors we must emphasize the importance of diet, this is why it is a priority in our program to look at a patient with cancer as an individual and not as a pathology. Nutritional intervention should be implemented as soon as the cancer is diagnosed.
According to the patient’s needs, considering the type of cancer treatment (curative or palliative), the clinical conditions and nutritional status. In order to reduce or even reverse the deterioration of nutritional status, to improve in general and consequently improve the Quality of Life; a nutritional counseling in our program is conducted by a nutritionist in patients with chronic diseases and cancer.
The Balance between energy expenditure and energy consumption, is a balance of life and survival in these patients.
WHAT ARE FREE RADICALS?
Free Radicals are made continuously as a product of the normal metabolism of each cell and they are inactivated by a set of mechanisms (some by enzymes and others by entrapment). They are normal components of cells and tissues, a pool of FR exists particularly in each cell line and in some cell types they allow a better adaptation to their habitat. When raising or decreasing physiological concentrations of reactive oxygen species (EROS) they can carry a significant functional impairment. Atherosclerosis, aging and cancer to name a few.
The excess free radicals cause cellular DNA damage, this damage can range from the modification of a base in DNA molecules, to the rupture of a chain of DNA, mutations, activation of oncogenes and inactivation of suppressor genes and endothelial damage favoring metastasis.
In cancer treatment, the mechanisms exerted by most chemotherapeutic agents and ionizing radiation in the death of the tumor cell, pass through an increase in the generation of more free radicals leading to irreversible tissue damage, for which antioxidant treatment provides a therapeutic advantage.
Antioxidants improve the quality of life and survival of patients with cancer, so its use is part of our program as a therapeutic and not only preventive.
It is a potent oxidant. Bacteria, fungi and viruses have no or very little antioxidant enzymes in their cell membranes, without protection, ozone will penetrate the cell membrane causing it to rupture and die, healthy cells have antioxidant enzymes in their membranes and are not affected by ozone therapy.
Medical studies on ozone have shown benefits such as: improved wound healing, anti- inflammatory effect, improves the immune system response, and increases the release of oxygen to tissues that have low oxygen perfusion.
Ozone in dentistry has revolutionized its practice, its main use is to treat the cause of a problem not the symptom, basically it is use as preventive and protective: ozonized water in the pre-treatment to disinfect the oral cavity, and as treatment: in the form of ozonized water and ozone gas, using this two conditions, the dentist can disinfect perfectly the oral cavity, in addition the ozone gas can penetrate the dentinal tubules in the dentine, periodontal pockets and the accessory channels, where others cannot access.
The use of ozone steam therapy as an integrated treatment modality for cancer is based on the premise that cancer cells and indeed most microorganisms are anaerobic and therefore cannot thrive in an oxygen-rich environment. To enrich the blood with oxygen flow, decreases the likelihood that the cancer cells will survive. In addition to creating an inhospitable environment for cancer cells, enriching the blood with activated oxygen (or ozone) has the added benefit of free radical oxidation in the body, which acts as a natural cleansing agent.
Some of the most commonly cited benefits of ozone are:
To inactivate bacteria, viruses, fungi, yeasts and protozoa, stimulates the immune system, purify the blood and lymphatic system, improving circulation and removes arterial plaque, improves oxygen metabolism, forms peroxides; it has been observed that it can promote or favor tumor dissolution.
METALS LIKE MERCURY IN OUR TEETH
Not only the mercury vapor from amalgam is the sole factor that may contribute to the onset and progression of cancer, the other components of amalgams such as nickel are involved in this alteration, for these reason and alterations caused to the immune system, the removal of metals from the teeth of patients admitted to the program is an important part of the conditioning phase.
There is another oral pathology, common in patients with cancer, it is called cavitation, these cavitation, are absent areas from the jaw bone, which were incurred after the removal of teeth, these avascular areas are conducive to the proliferation of bacteria, becoming chronic infectious foci favoring chronic diseases including cancer, autologous PRGF treatment of these areas is considered in our treatment program.
CLEANING THE COLON
Colon hydrotherapy, is part of our program on the conditioning phase, in order to make a deeper detoxification, colon hydrotherapy is an effective method to cleanse colon feces without the use of drugs, it also has benefits such as: the release and elimination of toxins from the stool, improves the quality of the circulating blood, supports the immune system and helps to restore the pH of the body and feces.
Glutathione is a protein of critical importance in cancer patients, it has been demonstrated in previous studies that its deficiency can determine alterations in the DNA sequence and promoting gene expression or inhibit abnormal genes that suppress tumors; this is a treatment which is increasingly and more frequently used in conjunction with conventional treatments.
The level of Glutathione is affected by having a low diet of glutathione, pollution, radiation, stress, injury, trauma, aging and infectious process, all these conditions favor the loss of glutathione, and this reduction affects the cells aging process and cell death.
Our own cells are able to produce its own glutathione, to defend against everyday threats, it is a potent detoxifier for this reason it is more abundant in the liver, there are several ways to increase it, oral forms are not the best of choice, since its absorption in the intestine is very poor, a systemic intravenous infusion is the best choice.
Unfortunately, the indiscriminate and undirected use of this supplement is becoming more common, but an overdose of glutathione, can cause the same effect as its deficiency, for this reason, we evaluated the blood level of glutathione in order to correct its deficiencies more precisely.
1.3.2. Decrease of the Blocker Cell
Decrease of the Blockers Cells for the Activating Immune System to determine WHICH CELLS CAN BLOCK THE IMMUNE SYSTEM ACTIVATION
Regulatory T cells are lymphocytes that are produced and activated as a proper response to the activation of the immune system, the production and activation of this cell population is intended to stop the HYPERAROUSAL OF THE IMMUNE SYSTEM, which may be harmful to organs and tissues.
Regulatory T Lymphocytes are characterized by being CD4 + CD25 +, they are originated in the thymus, and its main function is to inhibit the immune response against self-antigens, and they are producers of Interleukin-10, this interleukin blocks the activation of macrophages and dendritic cells, it also inhibits the production of interleukin-12 by activated dendritic cells and macrophages, this interleukin-12 released by these cells, will activate the cytotoxic lymphocytes CD4 + and CD8 +.
The Regulatory T cells in the tumor microenvironment are common mediators of the evasion of tumor to the response, when dendritic cells present the specific antigen to the cytotoxic cells (CD4+lymphocytes) it could happen that at the same time the antigens will be presented to the regulatory T cells (inhibiting lymphocytes ) and thus activation of cytotoxic cells is inhibited or suppressed, for this reason it is important that before cell Immunotherapy, these regulatory T cells should be inhibited so they do not compete with cytotoxic cells, ensuring thus the activation of the antitumor immune response.
The reduction of this cell population before starting immunotherapy is accomplished in a simple and effective way, with minimal doses to ensure that no side effects will be present.
1.3.3. Circulating Tumor Load Reduction
The detection of these circulating tumor cells and their monitoring during follow-up and treatment in cancer is part of our program.
When patients are evaluated according to the number of circulating tumor cells, therapy becomes more targeted and personalized, plus they are our source of obtaining the antigens to be used for the activation and maturation of dendritic cells.
The identification and quantification of tumor cells in peripheral blood may be useful to constitute initial evidence of metastasis process and constitute a risk marker of tumor recurrence.
WHAT ARE CIRCULATING TUMOR CELLS (CTC)?
Circulating Tumor Cells (CTCs) were first described in 1869 in patients with cancer by Asworth. They are epithelial tumor cells that are already present in the peripheral blood of patients with cancer at an early stage of the disease. It was assumed for a long time, that their presence in the circulating blood meant progress of neoplastic disease and that it could be directly related to emergence of tumor metastasis.
But their true biological significance could not be established by their small numbers and for not having at the time the techniques that allow their isolation and identification. Current technological developments have shown that CTCs have genomic alterations characteristic of malignant cells, and rarely can they be found in peripheral blood of healthy people. It is estimated that only 1 of 105-106 CTCs present in peripheral blood can enter distant tissues from the primary tumor and only a small percentage of these cells will develop as metastatic disease.
In cancer patients, the control of release and the number of circulating tumor cells should be considered as part of the conventional treatment of these patients, unfortunately this resource for the diagnosis and monitoring of patient outcomes is wasted , in our program this study has several purposes, we use it as a marker for the initial assessment of patients, one part of treatment will be directed toward decreasing the population of circulating tumor cells, on the other hand they are an indicator of tumor activity and the risk of metastasis, and finally their determination and isolation are a source of self-antigens which are then used to guide the dendritic cells specifically and efficiently.
There are some products of natural origin that have been evaluated in vitro and which have proven effective in reducing or destroying these circulating epithelial cells, we test some of these products in vivo in cells of each patient to determine which of these products is more effective in cellular destruction specific for each patient, some of these products are amygdalin, vitamin C, selenium, melatonin, among others.
1.3.4. Oxidative Stress Improvement
Oxidative stress plays an important role in disease progression, particularly in cancer. A concentration of free radicals, disrupts this balance, the free radicals are atoms that have one electron altered and they become unstable and highly reactive, normally they are produced by the cells, but are rapidly inactivated by certain enzymes, the rising or decreased of reactive oxygen species (EROS) can produce certain cell functional alterations, such as atherosclerosis, aging and cancer, this are the best examples.
It can be seen therefore that FRs are formed under physiological conditions at rates controlled by the cell defense mechanisms. In this pathological situation the production increases substantially, thus entering the state of oxidative stress.
The factors leading to this situation are:
220.127.116.11 Chemicals: Increased heavy metals, xenobiotics, tobacco components
18.104.22.168 Drug: adtiamycin
22.214.171.124 Physical: ultraviolet radiation, hyperoxia.
126.96.36.199 Organic and metabolic: calorie diet, poor anti-oxidant diet, diabetes, inflammatory processes and trauma, phenomena of ischemia – re perfusion events and strenuous exercise.
TOXICITY OF FREE RADICALS
Damage to biomolecules that are determined by FR is implicated in the development or exacerbation of numerous processes:
DEFENSE SYSTEM AGAINST FREE RADICALS
Defense mechanisms can be external or exogenous and internal or endogenous, to promote and ensure correction of oxidative stress, the treatment will be directed and personalized, in our patients the levels glutathione are evaluated so they may receive effective treatment for correction of oxidative stress.
Products such as glutathione, ozone and vitamin C play an important role in maintaining balance in the oxidative system; the specific and direct administration assures better results, therefore avoiding overdosing and the occurrence of undesirable side effects
1.3.5. Personalized Targeted Therapy with Naturacetics:
At present time there are several known natural products named “naturacetics” or products from alternative medicine, which have demonstrated efficacy in destroying tumor cells when tested in vitro, and to enhance the immune response of the patients, however, combinations and over dosage, have antagonistic effects in some cases, namely that the risk of combining medications will neutralize the effect of the other causing on both to annul or perish its real effect. On the other hand, this overload of products increases the work that the liver has to perform for “detoxifying” our system in a normal form, the best way to contribute to the treatment in reducing tumor burden in a natural way or with natural products, is to measure directly the action of products on malignant cells individually and equally on each patient and to evaluate products that improve cellular immunity on the patient’s own lymphocytic cells, thereby guiding and customizing the most appropriate treatment for each patient, without the risk of nullifying or losing the effect of the products.
We evaluate 26 “naturacetics” products for each patient, so they customized their treatment, and do not cause liver overload.
1.4. Immune System Activation
This is the most important phase of our treatment. Cells are taken from the patient, a complex in-vitro cell activation process is performed and then injected back to the patient.
Our third phase is the phase of ACTIVATING THE INMUNE SYSTEM, this is performed with the activation and proliferation of cells of the immune system involved in the destruction of tumors, these processes are carried just “in vitro” and subsequently are applied to patients, patients do not present side effects or clinical events of rejection, because it is their “own cells” this is the most important phase of the program and this is where the cellular immunotherapy is held with the dendritic cells, activated cytotoxic lymphocytic cells, natural killer cells and a cocktail of autologous cytokine that self-activate the immune system,
Recent studies indicate that immunotherapy is among the most promising strategies of modern cancer therapy. Its aim is to strengthen the innate and acquired immunity, highly specific for tumor cells and low toxicity to healthy cells of the patient.
Cellular immunotherapy is being considered as the fourth line of conventional therapy for cancer patients, since it has demonstrated in certain patient groups that it is effective in delaying the progression, improving quality of life, and in some cases, to lower tumor lesions.
We evaluated the cellular immunotherapy in patients with various solid tumors, since the person’s immune system has the same components and the same activation pathways, therefore all patients, and not the tumors, should respond similarly to activation of the immune system, the difference between the various types of (tumors) neoplasms, are the proteins that they release, the inaccessible vascular sites where they are located, and the blocking conditions and systemic toxicity that each patient has individually; as we reviewed earlier, the improvement of these conditions, leaves the immune response ready, unobstructed, to be activated effectively and efficiently.
Each individual will respond differently to the activation of the immune system, these differences are essentially quantitative, namely that some patients produce a larger amount of interferon and interleukins, for example; while other patients produce less of these proteins and cytokines. This principle is that the results cannot be homogenized or similar between patients, the immune response will depend on these conditions, but we have observed that even patients that produce low amounts of these activating proteins, will develop an effective immune response. Each individual will respond differently to the activation of the immune system, these differences are essentially quantitative, namely that some patients produce a larger amount of interferon and interleukins, for example; while other patients produce less of these proteins and cytokines. This principle is that the results cannot be homogenized or similar between patients, the immune response will depend on these conditions, but we have observed that even patients that produce low amounts of these activating proteins, will develop an effective immune response.
Goal is to improve the general conditions of patients, improve the quality of life so that it will be reflected in the survival, equally a remission or the disease stability and the delay in progression of the same, are other our goals, for these reasons the results will be independent of each patient and the immune response depends on the patient’s own immune system.
1.4.1. Advance Cell Therapy
THE ADVANCED CELL IMMUNOTHERAPY OR CANCER VACCINATION, is a way to administer immune system cells activated in vitro where patients require efficient and sustained activation of the immune response to achieve combat on intratumoral or circulating malignant cells without causing damage to other types of cells in the body, it is a form of personalized and specific treatment for the molecular characteristics of tumors.
Activated Dendritic Cells with Atigens of Tumor Cells
Dendritic cells are special type of cells that carry out the presentation of tumor antigens to cytotoxic T lymphocytes, thereby these cells help the body destroy cancer cells.
In our program the dendritic cells are autologous, namely monocytes from patients are transformed into antigen presenting dendritic cells, and in this way each vaccine is personalized and it is specific.
The process to obtain them is complex, but in the laboratory monocytes are cultured with a variety of cytokines and other growth factors to create the differentiation of these cells into dendritic cells, signals from the tumor or best said, antigens of each tumor, is different in each patient, for this reason, our dendritic cells are stimulated and matured with antigens from each patient own cancer cells, this way the signal is obtained more direct and effectively.
Once the maturation of cells is obtained, they are introduced back into the patient subcutaneously near clusters of lymph nodes, thereby facilitating access of cells to the lymph node, which is the site where the antigen presentation is carried out.
Natural killer cells (NK Cells)
Natural Killer Lymphocytes or NK cells, are a sort of lymphocytes which are characterized by containing gramzyms and perforins, substances which result in death of the target cells, such as cancer cells and infected cells, these cells have the capacity of not requiring antigen presenting cells, like dendritic cells in order to act, these lymphocytes can recognize target cells and join them by a series of proteins, mainly MHC I, achieving the identification and destruction of (target) affected cells, without affecting the NK cells.
These lymphocytes are capable of acting in circulation, as part of our program their aim is to destroy circulating tumor cells basically, thereby decreasing tumor burden, delaying the appearance of metastases and keeping the disease in remission for longer period of time.
Activated Cytotoxic Lymphocytes (LAK CELLS)
Cytotoxic lymphocytes and natural killer (NK) cells are specialized cells that lyse or destroy target cells, these cells can be infected cells or cancer cells, these cells recognize an outer portion of these cells so they release a proteinthat begins perforating the cell membrane called “perforin” killing cells and secondly they release other proteins that change the internal pH of target cells called “gramzyms” thereby causing cell death. Other more complex mechanism is activating a cascade of proteins called “caspases”, that at the end their activation they cause apoptosis or target cell death.
The activation of these cells is what makes them effective, there are various trigger mechanisms but the most used are cytokines or the binding to dendritic cells.
The use of these cells is referred to as Adoptive Cell Therapy, which is an important part of our program. Patients receive a combination of dendritic cells, activated cytotoxic cells and natural killer cells thereby potentiating the antitumor response of each patient.
1.4.2. Rejuvenate of Immune System Activation
The adjuvants are external or internal substances that help or potentiate the Immune Response, they can be proteins, cytokines or certain products that have the ability to trigger the immune response, but their individual application is not effective, for this reason it is added to the Cell Therapy phase, with the aim of increasing the effectiveness of the Immune Response.
How does Iscador work in the body of a person affected by cancer?
Research studies reveal, among others, the following effects:
The sum of these effects, proven by scientific observation, lead to the increased in the quality of life for the person with cancer, which translates clinically (what the patient experiences) in:
The side effects that occur with the administration of Iscador, are as common as the administration of any medication: allergic and pseudo- allergic reactions (Urticaria, Rash, etc.), fortunately they are extremely rare. Similarly, in rare cases it can heightened the symptoms of phlebitis (inflammation of veins), mainly associated with veins in which previously chemotherapy was applied. This is temporary, is not significant and it has never been a reason to reduce the dose of Iscador
Interferon is an important protein for the activation of cytotoxic cells, it has been evaluated by a couple of decades in patients with certain types of tumors and infectious processes with good results, and it is a good adjuvant for the Immune Response.
The Interferon has demonstrated to decrease the expression of angiogenic factors (factors that promote vascularization of tumors), thus influencing on the delay in the appearance of metastases. When lymphocytes are activated, they produce and release more interferon, thus maintaining for a longer time the activation of the immune system.
1.4.3. Located Epigenetics Therapy
What is Epigenetics?
The Epigenetics, is a branch of genetics that studies the “epigenetic” changes in cancer, in other words, it studies the changes that affect the gene sequence without altering its structure, but it does modify the expression of genes, this is important because the epigenetic ambient can be capable of blocking or inhibiting the expression of the tumor- suppressing genes, or the genes control tumors.
We approach this as an important part of the comprehensive treatment of patients with cancer that receive immune cell therapy, by combining the therapeutic strategy that favors the release of the genes that suppress tumors, this may help for a control and remission of tumors in a most effective way.
There are several products currently evaluated and approved for the epigenetic treatment, DNA methyltransferase (DNMT)blockers like procaine, which have shown that they can revert the aberrant mutations of DNA and that are associated with the occurrence of tumors, such as breast, liver, lung and nasopharyngeal cancer.
We also studied the effect when combined with certain cytotoxic agents such as cisplatin that demonstrated an increased therapeutic rate, and improved antitumor activity.
After the treatment at our center the patient is instructed to continue at home with directions to help you maintain immune system activation
And finally our last phase is MAINTENANCE, at this stage patient continue activating their immune system as directed with autologous cytokines and maintaining the balance of cell proliferation with natural agents that demonstrated “in vitro” effect on the destruction of circulating malignant cells.
Cellular Immunotherapy program is an intense activation of the immune system, this activation phase has a defined period of action and activation, after this period which can vary from 14 to 21 days, the immune system returns to its basal state, our goal is to maintain activation by a longer period of time, it is necessary that patients continue with products that are “immunogens” capable of activating the immune system, with specific diet and with a therapy of personalized supplement naturacetics.
1.5.1. Extension and reinforcement of Immune System Activation
SOLUBLE PRODUCTS OF THE AUTOLOGOUS LYMPHOCYTES
When lymphocytes are activated, they release a number of cytokines and proteins that in turn will activate other effector cells, thus firing the activation of the immune response. These cytokines are released to the outside of the cell and can be processed and purified for self- application in a safe and effective way.
This effector molecules or cytokines when produced by activated cells have different functions in each of the different cell populations, so we have:
INF Ϫ (gamma interferon): is produced by TH1 and cytotoxic lymphocytes, inhibits the growth of Th2 lymphocytes, produces macrophage activation and increases the expression of MHC class I and II, activates NK cells.
IL-2 (Interleukin-2): is produced by TH0, TH1 cells and some cytotoxic T lymphocytes, stimulates the growth of T cells and NK cells.
TNFα (Tumor Necrosing Factor alpha): is produced by TH1, some TH2 and cytotoxic T lymphocytes, it activates macrophages and induces the production of NO, it can activate the microvascular endothelium.
GM-CSF (GM Growth Factor): is produced by TH1 , some TH2 and cytotoxic T lymphocytes it promotes differentiation of B cells, inhibits the growth of T cells, it activates macrophages, induces differentiation into dendritic cells, on hematopoietic cells it increases the production of granulocytes, macrophages and dendritic cells.
Other biological effects have been demonstrated of these products such as:
1.5.2. Specific Nutrition Therapy for Patients with Cancer
NUTRITIONAL ASPECT IN PATIENTSWITHCANCER
Tumor cachexia or malnutrition has a profound impact on the physical, psychological and social development of these patients, because they have an altered metabolism, marked by an increase wastage of protein and fat, causing the loss of muscle mass, additionally these patients carbohydrate metabolism is modified by the rate at which tumors develop and grow with decreased hepatic glucose production and decreased insulin sensitivity in peripheral tissues.
These alterations contribute to increased energy expenditure and may lead to a progressive loss of metabolism and muscle mass, however, despite the hyper metabolism and weight loss for these patients can be exacerbated by increased stress or pain, infection and surgeries. Nutrient intake in these patients is diminished oral most canceled due to the side effects of chemotherapy, and radiation, all these conditions promote malnutrition affecting survival of patients.
Our program considers important the nutritional status of the patients, this has an important role in the activation of the immune response, patients receive specific counselling by trained personnel for this special conditions, with the objective that the patient will make better changes in their nutritional habits, oriented to prevention or correction of malnutrition, and improve in an indirect form the activation of the immune system.
Patients also receive an orientation of the food groups that have demonstrated benefits in patients with cancer and also receive an orientation to the food groups that should be prohibited or eliminated from their diet.
1.6.1. As is well known, the immune system of patients respond individually and is different in each of the patients, therefore each patient will respond or have different clinical response to therapy, each patient is individually and personally evaluated with their circulating cell count, the amount of lymphocytic Regulatory T cells, among other studies.
We evaluated 29 patients with various types of solid tumors receiving Cellular Immunotherapy, the blue bars show the amount of CTC (Circulating Tumor Cells) that patients had in their circulation at the time of arrival to the program, the red bars show the count of CTC patients presented at the end of Autologous Immunotherapy. Patient 13 and patient 21, showed an increase on this cells after treatment, both of which were from patients who had recently undergone chemotherapy and were both hematological malignancies, which makes evaluation somewhat difficult with this method, these cases especially were evaluated with blood blast sand in both there was a decrease.
Our assessment of effectiveness is based directly in vitro, with the patient’s own cells dynamically alive, where morphologically they are evaluated as specific cells and that these cells also release proteins or substances that indicate they are activated these substances are measured in the laboratory thereby we confirm their activation.
Patients are evaluated with the Scale RECISTR a dialogically with:
The graphic shows the expansion of cytotoxic lymphocytes incubated with I L- 2 from our cultures, the blue lines shows the level of total lymphocytes that patients had on admission to the cellular immunotherapy program, the red line indicates cell proliferation at 24 hours of culture, and finally the green line indicates cell expansion at 48 hours of culture, showing that the immune system of cancer patients respond to activation with cytokines in vitro environments.
2.1. Cancer Preventive Program
Did you know that one third of the most common types of cancer can be prevented?
In recent years the frequency of cancer has been increasing, there are more external factors that lead to its occurrence, prevention and therapeutic tool is a useful intervention to reduce their frequency. The change in lifestyle and changing the “modifiable factors” extend personal and family well-being.
The preventive program is created for families of cancer patients that are at risk of developing a malignancy and for people who do not have any risk factors but who want to improve their lifestyle. Our program is oriented General Prevention, basically identifying.
Chronic Inflammation and its control. The chronic inflammation is closely related to the risk of developing cancer.
Cancer Preventive Program Includes:
1. Identify existing chronic inflammation by laboratory studies.
2. Anti-inflammatory diet guide. Dental evaluation.
3. Improve balance oxidative and anti-inflammatory treatment.
Chronic Inflammation is a process that is triggered by the presence of viral, bacterial, parasitic infections, tissue injury or chronic stimulation of the tissues and in some cases not detectable and therefore untreated. Chronic inflammation causes oxygen free radicals, these radicals can act as antimicrobial agents, but unfortunately cause damage to DNA of cells, increasing the risk of mutations and carcinogenesis.
During the inflammatory process released various types of cytokines, among them is the Tumor Necrosis Factor, this is related to tumor promotion, the erythrocyte sedimentation rate and PCR are other studies that help early diagnosis of Chronic Inflammation.
Ozone as anti-inflammatory
Ozone is a potent anti-inflammatory, is involved in maintaining the oxidative balance, regulates metabolism, modulates the immune system, improves oxygen metabolism, is an effective anti-inflammatory of the general circulatory system. Chronic exposure to toxic metals and some toxics found in the preparation of foods contribute to prolong the inflammatory response.